Primer

Part:BBa_K5427017:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


RV pET22b+ Keratin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 29
    Illegal XbaI site found at 12
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 29
    Illegal NotI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 29
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 29
    Illegal XbaI site found at 12
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 29
    Illegal XbaI site found at 12
    Illegal XbaI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reverse primer for linearization of pET22b+ vector with keratin31.


Source

Not applicable.

References